ACETOACETATE FORMATION IN LIVER
نویسندگان
چکیده
منابع مشابه
Acetoacetate Formation in Liver
In 1944, Weinhouse et al. (1) reported that the incubation of carboxyllabeled octanoate with rat liver slices yielded acetoacetate in which the carboxyl and carbonyl carbons were equally labeled. Random condensation of 2-carbon units resulting from ,8 oxidation was therefore offered by these workers as a possible mechanism for the formation of acetoacetic acid from octanoate. The conversion of ...
متن کاملSynthesis of acetoacetate by liver enzymes.
Two mechanisms have been proposed for the formation of acetoacetate from acetyl-CoA by liver preparations. With the use of a partially purified system prepared from acetone powders of beef liver, Lynen et al. (1) obtained evidence that acetoacetate formation proceeded via P-hydroxy-fi-methylglutaryl coensyme A as intermediate and involved the HlMG-COAT condensing and HMG-Coh cleavage enzymes as...
متن کاملEnzymes involved in acetoacetate formation in various bovine tissues.
1. The activities of acetoacetyl-CoA thiolase, hydroxymethylglutaryl-CoA synthase and lyase and acetoacetyl-CoA deacylase were measured in homogenates of samples of liver, rumen epithelium (long papillae), kidney and lactating mammary gland derived from slaughtered cows. 2. The activities of the four enzymes in bovine liver were similar to the activities previously reported for the correspondin...
متن کاملEnzymatic condensation of acetate to acetoacetate in liver extracts.
* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Le...
متن کاملFormation of acetoacetate from fatty acids by particulate systems of rat liver.
The relative importance of oxidation through the Krebs cycle or of conversion to acetoacetate for the oxidation of fatty acids in liver is unknown. Attempts have been made to answer this question by studies of the products of the oxidation of fatty acids in particulate systems (l-7). In most of the experiments this process has required the presence not only of adenine nucleotides, magnesium ion...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)65480-8